| CHAPTER | CHARTER YEAR | CITY/SEAT | TYPE | AREA |
|---|
| Epsilon Mu Lambda | 1951 | Pensacola | Alumni | Northwest |
| Rho Omicron Lambda | 2004 | Ft. Walton Beach | Alumni | Northwest |
| Mu Theta | 1976 | University of West Florida | College | Northwest |
| Epsilon Pi Lambda | 1952 | Ocala | Alumni | North |
| Gamma Mu Lambda | 1946 | Tallahassee | Alumni | North |
| Nu Eta Lambda | 1979 | Gainesville | Alumni | North |
| Pi Psi Lambda | 1994 | Quincy | Alumni | North |
| Sigma Pi Lambda | 2019 | Orange Park | Alumni | North |
| Upsilon Lambda | 1925 | Jacksonville | Alumni | North |
| Beta Nu | 1932 | Florida A&M University | College | North |
| Iota Delta | 1974 | Florida State University | College | North |
| Kappa Upsilon | 1976 | Edward Waters University / Jacksonville University | College | North |
| Sigma Upsilon | 1993 | University of North Florida | College | North |
| Theta Sigma | 1972 | University of Florida | College | North |
| Beta Delta Lambda | 1937 | Daytona | Alumni | East |
| Delta Xi Lambda | 1953 | Orlando | Alumni | East |
| Iota Beta Lambda | 1967 | Cocoa | Alumni | East |
| Delta Beta | 1948 | Bethune Cookman University | College | East |
| Xi Iota | 1979 | University of Central Florida | College | East |
| Gamma Zeta Lambda | 1945 | Tampa | Alumni | West |
| Mu Zeta Lambda | 1976 | Lakeland | Alumni | West |
| Omicron Beta Lambda | 1985 | Clearwater | Alumni | West |
| Theta Eta Lambda | 1962 | Saint Petersburg | Alumni | West |
| Xi Omicron Lambda | 1983 | Fort Myers | Alumni | West |
| Xi Psi Lambda | 1984 | Palmetto | Alumni | West |
| Rho Xi | 1990 | Saint Leo University | College | West |
| Theta Gamma | 1972 | University of South Florida | College | West |
| Tau Pi | 2003 | University of Tampa | College | West |
| Upsilon Sigma | 2015 | Florida Gulf Coast University | College | West |
| Beta Beta Lambda | 1937 | Miami | Alumni | South |
| Delta Delta Lambda | 1947 | West Palm Beach | Alumni | South |
| Eta Kappa Lambda | 1960 | Fort Pierce | Alumni | South |
| Iota Pi Lambda | 1970 | Richmond Heights | Alumni | South |
| Omicron Upsilon Lambda | 1987 | Delray Beach | Alumni | South |
| Zeta Alpha Lambda | 1954 | Fort Lauderdale | Alumni | South |
| Delta Psi | 1954 | Florida Memorial University | College | South |
| Eta Delta | 1970 | University of Miami | College | South |
| Tau Delta | 2001 | Florida International University | College | South |
| Upsilon Theta | 2011 | Florida Atlantic University | College | South |
| Iota Epsilon Lambda | 1977 | Nassau, Bahamas | Alumni | The Bahamas |
| Sigma Beta Lambda | 2011 | Freeport, Bahamas | Alumni | The Bahamas |
| Phi Mu | 2019 | University of the Bahamas | College | The Bahamas |
| Iota Sigma Lambda | 1971 | St. Croix, US Virgin Islands | Alumni | Virgin Islands |
| Theta Epsilon Lambda | 1962 | St. Thomas, Virgin Islands | Alumni | Virgin Islands |
| Tau Beta Lambda | 2021 | Petion Ville, Haiti | Alumni | Haiti |
| Tau Delta Lambda | 2021 | San Juan, Puerto Rico | Alumni | Puerto Rico |
| Tau Zeta Lambda | 2021 | Santo Domingo, Dominican Republic | Alumni | Dominican Republic |
| Tau Kappa Lambda | 2023 | Panama City, Panama | Alumni | Panama |
