| CHAPTER | CITY/SEAT | TYPE | AREA |
|---|
| Epsilon Mu Lambda | Pensacola | Alumni | Northwest |
| Rho Omicron Lambda | Ft. Walton Beach | Alumni | Northwest |
| Mu Theta | University of West Florida | College | Northwest |
| Epsilon Pi Lambda | Ocala | Alumni | North |
| Gamma Mu Lambda | Tallahassee | Alumni | North |
| Nu Eta Lambda | Gainesville | Alumni | North |
| Pi Psi Lambda | Quincy | Alumni | North |
| Sigma Pi Lambda | Orange Park | Alumni | North |
| Upsilon Lambda | Jacksonville | Alumni | North |
| Beta Nu | Florida A&M University | College | North |
| Iota Delta | Florida State University | College | North |
| Kappa Upsilon | Edward Waters University / Jacksonville University | College | North |
| Sigma Upsilon | University of North Florida | College | North |
| Theta Sigma | University of Florida | College | North |
| Beta Delta Lambda | Daytona | Alumni | East |
| Delta Xi Lambda | Orlando | Alumni | East |
| Iota Beta Lambda | Cocoa | Alumni | East |
| Delta Beta | Bethune Cookman University | College | East |
| Xi Iota | University of Central Florida | College | East |
| Gamma Zeta Lambda | Tampa | Alumni | West |
| Mu Zeta Lambda | Lakeland | Alumni | West |
| Omicron Beta Lambda | Clearwater | Alumni | West |
| Theta Eta Lambda | Saint Petersburg | Alumni | West |
| Xi Omicron Lambda | Fort Myers | Alumni | West |
| Xi Psi Lambda | Palmetto | Alumni | West |
| Rho Xi | Saint Leo University | College | West |
| Theta Gamma | University of South Florida | College | West |
| Tau Pi | University of Tampa | College | West |
| Upsilon Sigma | Florida Gulf Coast University | College | West |
| Beta Beta Lambda | Miami | Alumni | South |
| Delta Delta Lambda | West Palm Beach | Alumni | South |
| Eta Kappa Lambda | Fort Pierce | Alumni | South |
| Iota Pi Lambda | Richmond Heights | Alumni | South |
| Omicron Upsilon Lambda | Delray Beach | Alumni | South |
| Zeta Alpha Lambda | Fort Lauderdale | Alumni | South |
| Delta Psi | Florida Memorial University | College | South |
| Eta Delta | University of Miami | College | South |
| Tau Delta | Florida International University | College | South |
| Upsilon Theta | Florida Atlantic University | College | South |
| Iota Epsilon Lambda | Nassau, Bahamas | Alumni | Bahamas |
| Sigma Beta Lambda | Freeport, Bahamas | Alumni | Bahamas |
| Phi Mu | University of the Bahamas | College | Bahamas |
| Iota Sigma Lambda | St. Croix, US Virgin Islands | Alumni | Virgin Islands |
| Theta Epsilon Lambda | St. Thomas, Virgin Islands | Alumni | Virgin Islands |
| Tau Beta Lambda | Petion Ville, Haiti | Alumni | Haiti |
| Tau Delta Lambda | San Juan, Puerto Rico | Alumni | Puerto Rico |
| Tau Zeta Lambda | Santo Domingo, Dominican Republic | Alumni | Dominican Republic |
| Tau Kappa Lambda | Panama City, Panama | Alumni | Panama |
